Anti-Angiopoietin 2/ANGPT2 Antibody

Anti-Angiopoietin 2/ANGPT2 Antibody

• Background: ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
• Description: Boster Bio Anti-Angiopoietin 2/ANGPT2 Antibody catalog # RP1037. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
• Synonyms: Angiopoietin-2;ANG-2;ANGPT2;
• Gene Name: Angiopoietin-2
• Gene ID: 285
• UniProt: O15123
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human Angiopoietin 2 recombinant protein (Position: Y19-N348). Human Angiopoietin 2 shares 84% and 85% amino acid (aa) sequences identity with mouse and rat Angiopoietin 2, respectively.
• Clonality: Polyclonal
• Applications: IHC
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• References & Citations: 1. Bhandari, V., Choo-Wing, R., Lee, C. G., Zhu, Z., Nedrelow, J. H., Chupp, G. L., Zhang, X., Matthay, M. A., Ware, L. B., Homer, R. J., Lee, P. J., Geick, A., de Fougerolles, A. R., Elias, J. A. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death. Nature Med. 12: 1286-1293, 2006. 2. Kim, I., Kim, J.-H., Ryu, Y. S., Jung, S. H., Nah, J. J., Koh, G. Y. Characterization and expression of a novel alternatively spliced human angiopoietin-2. J. Biol. Chem. 275: 18550-18556, 2000. 3. Cheung AH, Stewart RJ, Marsden PA (Jun 1998). "Endothelial Tie2/Tek ligands angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23". Genomics 48 (3): 389–91. 4. Fiedler, Ulrike; Krissl Tanja, Koidl Stefanie, Weiss Cornelia, Koblizek Thomas, Deutsch Urban, Martiny-Baron Georg, Marmé Dieter, Augustin Hellmut G (Jan 2003). "Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats". J. Biol. Chem. (United States) 278 (3): 1721–7.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 56919 MW
• Observed Molecular Weight: 125 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  
• Subcellular Location: Secreted.
• Sequence Similarities: Contains 1 fibrinogen C-terminal domain.
• Protein Name: Angiopoietin-2