Anti-GAPDHS Purified Monoclonal Antibody

Anti-GAPDHS Purified Monoclonal Antibody

• Background: GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1, 3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
• Description: Boster Bio Anti-GAPDHS Purified Monoclonal Antibody (Catalog# M06121). Tested in WB, ICC, Flow Cytometry application(s). This antibody reacts with Pig, Human.
• Synonyms: SGAPDH, HSD-35
• CAS Number: 9007-83-4
• Gene Name: GAPDHS
• UniProt: O14556
• Host: Mouse
• Reactivity: Pig, Human
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
• Clonality: Monoclonal
• Applications: WB, ICC, Flow Cytometry
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Concentration: 1 mg/ml
• Form: Liquid
• Molecular Weight: 44501 MW
• Components: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Storage Conditions: Store at 2-8°C. Do not freeze.
• Fragment: Mouse IgM
• Clone ID: Clone: Hs-8