Anti-Bub1 Antibody

Anti-Bub1 Antibody

• Background: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
• Description: Boster Bio Anti-Bub1 Antibody (Catalog # A00776). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
• Synonyms: BUB1A, BUB1L, hBUB1, BUB1A
• CAS Number: 9007-83-4
• Gene Name: BUB1
• UniProt: O43683
• Host: Rabbit
• Reactivity: Human, Mouse
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 - 90 of Bub1.
• Clonality: Polyclonal
• Applications: ELISA, WB, ICC, IF
• Purification: Bub1 Antibody is affinity chromatography purified via peptide column.
• Concentration: 1 mg/mL
• Form: Liquid
• Molecular Weight: 122375 MW
• Components: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Storage Conditions: Bub1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Fragment: IgG