Recombinant Mouse Interleukin-6/IL-6 Protein

• Background: Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
• Abbreviation: Interleukin-6, IL-6
• Synonyms: BSF; Interleukin; Interleukin HP; IFNB; B-cell hybridoma growth factor; BSF2; CDF; HGF; HSF; IFNB2; IL 6; Interleukin HP-1; Interleukin-6; Il-6; Il6
• UniProt: P08505
• Accession Number: P08505
• Expression System: E. coli
• Tag: C-His
• Sequence: Phe25-Thr211
• Applications: Cell culture
• Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce proliferation in 7TD1 cells. The ED₅₀ for this effect is <0.01 ng/mL. The specific activity of recombinant mouse IL-6 is approximately >1x 10⁸ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Calculated Molecular Weight: 22.7 kDa
• Observed Molecular Weight: 24 kDa
• Formulation: Lyophilized from sterile PBS, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.
• Species: Mouse