Recombinant Mouse B7-DC/PD-L2/CD273 Protein (Fc Tag)

Recombinant Mouse B7-DC/PD-L2/CD273 Protein (Fc Tag)

• Background: Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
• Synonyms: B7-DC, Btdc, CD273, F730015O22Rik, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Pdcd1lg2, Programmed cell death 1 ligand 2
• CAS Number: 9000-83-3
• UniProt: Q9WUL5
• Accession Number: Q9WUL5
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-hFc
• Sequence: Met 1-Arg 219
• Applications: ELISA
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized human PD1-His at 10 μg/mL (100 μl/well) can bind mouse PD-L2-Fc, The EC50 of mouse PD-L2-Fc is 0.95 μg/mL.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 60 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.