QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit

QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit

• Abbreviation: IGF-1
• Synonyms: insulin-like growth factor 1;IGF1;
• CAS Number: 7732-18-5
• UniProt: PN/A5N/A19
• Species Reactivity: Human
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cancer;Developmental biology;Signal transduction
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF-1 are added to the micro ELISA plate wells. Human IGF-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGF-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IGF-1. The concentration of Human IGF-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Detection Range: 3.12-100 ng/mL
• Sensitivity: 1.01 ng/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months