Mouse anti Human LEF-1 beta catenin

Mouse anti Human LEF-1 beta catenin

• Background: Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on Western blots as smaller immunoreactive forms.
• Synonyms: lef-1, Mouse Anti Human lymphoid enhancer factor-1
• CAS Number: 9007-83-4
• UniProt: Q9UJU2
• Host: Mouse
• Species Reactivity: Human, Mouse
• Isotype: IgG1
• Clone: REMB1
• Conjugation: Unconjugated
• Type: Monoclonal Antibody
• Source: The immunogen used for production of the mouse monoclonal anti-LEF-1 antibody was a full length protein.
• Applications: WB, IF, IHC (paraffin)
• Field of Research: Signal Transduction
• Purification Method: Protein A/G Chromatography
• Assay Principle: Antibody can be used for Western blotting (1-5 µg/mL), immunofluorescence and in immunohistochemistry on paraformaldehyde-fixed cells and paraffin embedded tissues (1:1000 or 1 µg/mL). Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Clone REMB1 recognizes the beta-catenin binding domain of LEF-1. Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: Hsu SC; Galceran J; Grosschedl R. 'Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.' Mol Cell Biol, 18(8):4807-18 1998_x000D_ IHC Referene: Dorfman, David M. et al. , Loss of Expression of the WNT/Beta Catenin Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell factor-1 (TCF-10 in a Subset of Peripheral T Cel Lymphomas, The American Journal of Pathology, 2003, pp 1538-1544.
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB