Mouse anti Human Insulin

Mouse anti Human Insulin

• Background: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
• CAS Number: 9007-83-4
• UniProt: P01308
• Host: Mouse
• Species Reactivity: Human, Bovine, Porcine
• Isotype: IgG1
• Clone: E2E3
• Conjugation: Unconjugated
• Type: Monoclonal Antibody
• Applications: WB, ELISA, IHC
• Field of Research: Signal Transduction
• Purification Method: Protein A/G Chromatography
• Assay Principle: Antibody can be used for Western blotting (1-5 µg/mL), immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 µg/mL, incubate at room temp. for at least 1 hr.) and ELISA. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Roth, J., et al. 'Distribution patterns of proinsulin and insulin in Human insulinomas: an immunohistochemical analysis in 76 tumors.' Virchows Arch B Cell Pathol Incl Mol Pathol 63, 51-61 (1992). 2. Nykiforuk, C.L., et al. 'Transgenic expression and recovery of biologically active recombinant Human insulin from Arabidopsis thaliana seeds.' Plant Biotechnology J. 4, 77-85 (2006) 3. Lee, A.H., et al. 'XBP-1 is required for biogenesis of cellular secretory machinery of exocrine glands.' EMBO J., 24, 4368-4380 (2005)
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB