Rabbit Anti-Mouse VEGF-A Polyclonal Antibody

Rabbit Anti-Mouse VEGF-A Polyclonal Antibody

• Description: Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains.
• Synonyms: Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188
• CAS Number: 9007-83-4
• NCBI Gene ID: 22339
• UniProt: Q00731
• Accession Number: NP_001020421
• Accession Number mRNA: NM_001025250
• Gene Location: 17C; 17 24.2cM
• Host: Rabbit
• Origin Species: Mouse
• Species Reactivity: Mouse
• Target Antigen: Recombinant Mouse VEGF164 (RT #M30-001)
• Clone: Rabbit IgG
• Validated Applications: WB, IHC (P), ELISA
• Purification Method: Protein A purified
• Detection Range: Western Blot: use at 2-5 µg/ml.
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.