Mouse Anti-Human ALK2 (Activin R1A) Monoclonal Antibody

Mouse Anti-Human ALK2 (Activin R1A) Monoclonal Antibody

• Description: Activin RIA, also known as ALK2, TSK7L, SKR1, TSRI, and ACTRI, is a glycosylated 65 kDa type I receptor in the TGFβ serine/threonine kinase receptor family. Binding of TGFβ superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins. Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis. Mature human Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Serrich GS box and the kinase domain. Within the ECD, human Activin RIA shares 99% and 94% aa sequence identity with mouse and rat Activin RIA, respectively. It shares 20% 26% aa sequence identity with other human type I receptors Activin RIB, BMPRIA, BMPRIB, and TGFβ RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGMB/ DRAGON, a BMP coreceptor that also associates with other type I and type II receptors. Activin RIA can additionally phosphorylate the coreceptor Endoglin and is required for the inhibitory effect of Endoglin on prostate cancer cell motility.
• Synonyms: ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• CAS Number: 9007-83-4
• NCBI Gene ID: 90
• UniProt: Q074771
• Accession Number: NP_001096.1
• Accession Number mRNA: NM_001105
• Gene Location: 2q23-q24
• Host: Mouse
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: Recombinant Human ALK2 extracellular domain
• Isotype: IgG1
• Clone: (#9J17)
• Validated Applications: WB, IHC
• Purification Method: Protein G chromatography
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.