Anti-NGFR (Nerve Growth Factor Receptor)(NGFR5), CF740 conjugate
CAT:
37-BNC740890-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-NGFR (Nerve Growth Factor Receptor)(NGFR5), CF740 conjugate
- Description: This antibody recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
- Synonyms: CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor (NGFR); p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: NGFR
- Gene ID: 4804
- NCBI Gene ID: 415768; 681726
- UniProt: P08138
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Baboon, Cat, Ferret, Human, Monkey, Rabbit
- Immunogen: NGFR from A875 melanoma cells
- Target Antigen: NGFR
- Clonality: Monoclonal
- Isotype: IgG1 κ
- Clone: NGFR5
- Conjugation: CF740
- Disease: Tumor
- Source: Animal
- Applications: IHC, FFPE (verified)
- Validated Applications: IHC, FFPE
- Field of Research: Cancer, Developmental biology, Signal transduction
- Positive Control: Melanoma|Neuronal axons, Schwann cells, and perineural cells of peripheral nerves|Soma and axons of sensory neurons, and ganglionic satellite cells|Tumors of nerve sheath differentiation, Schwannoma, Neurofibroma
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% rBSA, 0.05% azide
- Molecular Weight: 75 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years