Anti-Alkaline Phosphatase(V17.1), CF740 conjugate

Anti-Alkaline Phosphatase(V17.1), CF740 conjugate

• Description: This antibody reacts with tissue non-specific alkaline phosphatase. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Alkaline phosphatase tissue non-specific; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase biomineralization associated; TNAP; TNALP; ALPL
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ALPP
• Gene ID: 248
• NCBI Gene ID: 284255; 333509; 370099; 75431
• UniProt: P05186; P05187; P09923; P10696
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Cow, Human
• Immunogen: Bovine intestinal alkaline phosphatase
• Target Antigen: Alkaline Phosphatase
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: V17.1
• Conjugation: CF740
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Developmental biology, Metabolism
• Positive Control: Intestine
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 55 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (frozen) 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years