Anti-Bov CD18 Alexa Fluor® 488

Anti-Bov CD18 Alexa Fluor® 488

• Background: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1 severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
• Specifications: The antibody IVA35 reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Human
• Immunogen: Bovine leukocytes.
• Target Antigen: CD18
• Isotype: IgG1
• Clone: IVA35
• Applications: Flow cytometry: Recommended dilution: 2-5 µg/mL
• Validated Applications: ICC, FC
• Concentration: 0.5 mg/mL
• Format: Alexa Fluor® 488
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Naessens J & Hopkins J Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204?dopt=A,*Dusinsky R, Tomaskova J, Horovska L, Simon M: Monoclonal antibodies specific for bovine CD18. Folia Biol (Praha). 200147(3):108-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/11409317?dopt=,*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076?dopt=A,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.