Mouse anti Human CD2 FITC - CD7 PE

Mouse anti Human CD2 FITC - CD7 PE

• Background: Identification of Human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen. Identification of Human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cell. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.
• Synonyms: CD2 FITC - CD7 PE
• CAS Number: 9007-83-4
• UniProt: P06729, P09564
• Host: Mouse
• Species Reactivity: Human
• Conjugation: Bi-Test (FITC/RPE) Reagent
• Type: Bi-Testª Reagents (FITC/RPE)
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB /100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Stability: See expiration date on vial
• Concentration: Titered for flow cytometry
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1.An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131 2.Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class. Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):85419 3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785 4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20 5. Association of CD2 and CD45 on Human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 6. Isolation and characterization of the genomic Human CD7 gene: structural similarity with the murine Thy-1 gene. Schanberg LE, Fleener DE, Kurtzberg J, Haynes BF, Kaufman RE; Proc Natl Acad Sci USA 1991 Jan 1;88(2):603-7 7. Identification of novel B-lineage cells in Human fetal bone marrow that coexpress CD7. Grumayer ER, Griesinger F, Hummell DS, Brunning RD, Kersey JH; Blood 1991 Jan ; 77(1):64-8 8. Genuine CD7 expression in acute leukemic and lymphoblastic lymphoma. Osada H, Emi N, Ueda R, Seto M, Koike K, Suchi T, Kojima S, Obata Y, Takahashi T; Leuk Res 1990;14(10):869-77 9. Inhibition of alloresponsive naive and memory T cells by CD7 and CD25 antibodies and by cyclosporine. Akbar An, Amlot PL, Ivory K, Timms A, Janossy G; Transplantation 1990 No;50(5):823-9 10. Comparsion of outcome, clinical, laboratory, and immunological features in 164 children and adults with T-ALL. Garand R, Vannier JP, Bene MC, Faure G, Favre M, Bernard A; Leukemic 1990 No;4(11):739-44
• Shipping Conditions: Ambient Temperature, do not freeze, refrigerate upon arrival
• Storage Conditions: Product should be stored at 4-8ºC; DO NOT FREEZE
• Functional Analysis: Flow Cytometry Staining