Anti-BOB.1 (B-Cell Marker) (BOB1/2425)

• Description:

  BOB-1 is a transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2, boosting the promoter activity of those complexes. It is essential for the response of B-cells to antigens, required for the formation of germinal centers, and regilates IL-6 expression. BOB-1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  BOB-1; B-cell-specific coactivator OBF-1; OCT-binding factor 1; POU domain class 2-associating factor 1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  POU2AF1

• Gene ID:

  5450

• NCBI Gene ID:

  654525

• UniProt:

  Q16633

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human BOB1 (POU2AF1) protein (around aa 148-255) (exact sequence is proprietary)

• Target Antigen:

  BOB.1

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  BOB1/2425

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Immunology

• Positive Control:

  Raji and Ramos cells. Spleen or Tonsil.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  35 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years