Anti-Desmoglein-1 (DSG1) (18D4), CF640R conjugate

• Description:

  This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  DSG1

• Gene ID:

  1828

• NCBI Gene ID:

  2633

• UniProt:

  Q02413

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human desmoglein-1 intracellular domain (exact sequence is proprietary)

• Target Antigen:

  Desmoglein-1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  18D4

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  IF (published) | IHC, FFPE (published) | WB (published)

• Validated Applications:

  IF, IHC, FFPE, WB

• Field of Research:

  Cell adhesion

• Positive Control:

  A431 or A-375 cells. Skin

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  150 kDa (precursor); 160 kDa (mature)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. New England J Med (2000) 343:31-35. (IHC, FFPE) J Inv Dermatol (2001) 117(5): 1302-1306. (WB) J Biol Chem (2006) 281(6): 3614-3624. (WB) Front Immunol (2019) 10: 2635. (IF)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years