Anti-Testosterone(4E1G2), CF488A conjugate

• Description:

  This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone <0.1%, 5-alpha DHT 0.8%, estradiol <0.1% and progesterone <0.1%. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  17β-Hydroxy-3-oxo-4-androstene, 17β-Hydroxy-4-androsten-3-one, 4-Androsten-17β-ol-3-one, trans-Testosterone

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• UniProt:

  Not Applicable

• Cellular Locus:

  Cytoplasmic|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Pan species

• Immunogen:

  Testosterone 3 CMO conjugated to BSA

• Target Antigen:

  Testosterone

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  4E1G2

• Conjugation:

  CF488A

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | RIA (published)

• Validated Applications:

  IHC, FFPE, RIA

• Field of Research:

  Endocrinology

• Positive Control:

  Interstitial or Leydid cells in testis.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  288.42Da

• Additionnal Information:

  Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min|Immunohistology formalin-paraffin 0.25-0.5 ug/mL|ELISA For coating, order Ab without BSA|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Hum Reprod Sci (2018) 11(3): 291-296. (RIA) Syst Biol Reprod Med (2018) 64(3): 191-201. (RIA)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years