Caspase 3/7 and DAPI Double Staining Kit

CAT:

763-E-CK-A833-01

Size:

20 Assays

Price:

Ask

Type:
Cell Function Related Products
Detection Method:
Fluorometric method; Flow cytometry
Assay Principle:
The Caspase 3/7 Substrates (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD), which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. DAPI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods.
Assay Performance Time:
0.5 Hour
Sample Type:
Cell samples
Shipping Conditions:
Ice Bag
Storage Conditions:
This product can be stored at 2~8°C for 1 year in the dark.
Detection Instrument:
Flow Cytometer; Fluorescence Microscope

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