Anti-IGF-1(M23)

Anti-IGF-1(M23)

• Description: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Insulin-like Growth Factor IA (IGF-IA); Insulin-like Growth Factor IB (IGF-IB); Mechano Growth Factor (MGF); Somatomedin-C
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IGF1
• Gene ID: 3479
• NCBI Gene ID: 160562
• UniProt: P05019
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human, Mouse, Rabbit, Rat
• Immunogen: Purified human IGF-1 protein
• Target Antigen: IGF-1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: M23
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IHC, FFPE (published) | WB (published)
• Validated Applications: IHC, FFPE, WB
• Field of Research: Cancer, Signal transduction
• Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: ~7.6 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. In Vivo (2009) 23: 567-576. (IHC, FFPE; WB)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years