Anti-GM-CSF(BVD2-21C11)

• Description:

  Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Burst Promoting Activity; Eosinophil Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CSF2

• Gene ID:

  1437

• NCBI Gene ID:

  1349

• UniProt:

  P04141

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Rat

• Species Reactivity:

  Cynomolgus, Human, Rhesus macaque

• Immunogen:

  Recombinant human GM-CSF protein

• Target Antigen:

  GM-CSF

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  BVD2-21C11

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  Flow, intracellular (published) | IF (published) | ELISA (published)

• Validated Applications:

  FC, IF, ELISA

• Field of Research:

  Cytokines, Immunology

• Positive Control:

  Lymph node and tonsil

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  22 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunoprecipitation: 0.5-1 ug/500 ug protein lysate|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (frozen) 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Neutralization Studies order Ab without azide|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (2015) 194: 5085-5093. (Flow, intracellular; IF) J Exp Med (1999) 190 (6): 875-880. (ELISA)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years