Rat F1+2 (Prothrombin Fragment 1+2) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• CAS Number:

  7732-18-5

• Gene Aliases:

  F1+2

• Field of Research:

  Hematology.

• Assay Principle:

  Sandwich ELISA

• Assay Performance Time:

  3.5h

• Standard:

  200 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  3.13-200 ng/mL

• Sensitivity:

  1.36 ng/mL