Anti-Cyclin A2(E67), CF640R conjugate

• Description:

  This antibody recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located in amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_

• Synonyms:

  CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CCNA2

• Gene ID:

  890

• NCBI Gene ID:

  58974

• UniProt:

  P20248

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Cow, Green monkey, Human, Mink, Monkey, Mouse

• Immunogen:

  Full length bovine Cyclin A protein

• Target Antigen:

  Cyclin A2

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  E67

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cell cycle

• Positive Control:

  K-562 and HeLa Cells

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  54 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years