Dog CTXIII (Cross Linked C-Telopeptide Of Type III Collagen) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CTXIII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CTXIII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CTXIII in the samples is then determined by comparing the OD of the samples to the standard curve.

• CAS Number:

  7732-18-5

• Gene Aliases:

  CTX-III; CTX3

• Field of Research:

  Metabolic pathway; Nutrition metabolism; Bone metabolism.

• Assay Principle:

  Competitive Inhibition ELISA

• Assay Performance Time:

  2.5h

• Standard:

  10000 pg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  156.25-10000 pg/mL

• Sensitivity:

  45.8 pg/mL