Mouse Hyp (Hydroxyproline) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.

• CAS Number:

  7732-18-5

• Gene Aliases:

  (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

• Field of Research:

  Metabolic pathway; Nutrition metabolism.

• Assay Principle:

  Competitive Inhibition ELISA

• Assay Performance Time:

  2.5h

• Standard:

  5000 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  78.13-5000 ng/mL

• Sensitivity:

  27.3 ng/mL