Anti-CD84(153-4D9), CF488A conjugate

• Description:

  Recognizes a protein of 74 kDa, identified as CD84. It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cell surface antigen MAX.3; hCD84; Hly9-beta; Leucocyte differentiation antigen CD84; LY9B; Signaling lymphocytic activation molecule 5 (SLAMF5); SLAM family member 5

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD84

• Gene ID:

  8832

• NCBI Gene ID:

  398093

• UniProt:

  Q9UIB8

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  MLR-stimulated lymphocytes

• Target Antigen:

  CD84

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  153-4D9

• Conjugation:

  CF488A

• Source:

  Animal

• Applications:

  Flow (verified) | Flow, surface (published)

• Validated Applications:

  FC, FC,

• Field of Research:

  Immunology

• Positive Control:

  Raji, HL60, THP-1, HMC-1, HEL and NAMALWA cells. Human platelets and macrophages. Human tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  74 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, 1995. Blood (1997) 90(6): 2398-2405. (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years