Anti-CD7(T3-3A1), CF568 conjugate

• Description:

  Recognizes a protein of 40 kDa, identified as CD7, a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD27 ligand (CD27L); CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD7

• Gene ID:

  924

• NCBI Gene ID:

  186820

• UniProt:

  P09564

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human T cells

• Target Antigen:

  CD7

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  T3-3A1

• Conjugation:

  CF568

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IF (published) | ELISA (published) | IP (published)

• Validated Applications:

  FC, IF, ELISA, IP

• Field of Research:

  Immunology, Signal transduction

• Positive Control:

  Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  40 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1979) 76: 5829-5833. PNAS USA (1980) 77(5): 2914-2918. (IP; functional studies) J Immunol Meth (1987) 96(2), 201-209. (cell ELISA) PNAS USA (1996) 93: 10348-10353. (cell depletion)>/li> Blood (1998) 91(8) 2760-2771. (Flow) J Cell Biol (1998) 142(5): 1245-1256. (IF; IP) Theranostics (2018) 8(21): 6070-6087. (mAb characterization; in vivo imaging)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years