Anti-CD37(IPO-24)

Anti-CD37(IPO-24)

• Description: Recognizes a protein of 33-55 kDa, identified as CD37 (Workshop V; Code CD37.7). CD37 is strongly expressed on normal and neoplastic mature (sIg ) B-lymphocytes. In B-cell ontogeny, CD37 appears after the pre-B-cell stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells.1 CD37 is also present, at low densities, on resting and activated T cells, neutrophils, monocytes, and some myelomonocytic leukemia cells. It is absent from platelets, erythrocytes. CD37 is a member of a family of tetraspan transmembrane proteins, including CD9, CD53, CD63, CD81, and CD82. It associates other tetraspan transmembrane proteins and MHC class II molecules to form a large complex at the surface of B cells and play a role in signal transduction. CD37 is a valuable and stable marker for peripheral mature B-cells and corresponding malignancies like B-cell chronic lymphocytic leukemia (B-CLL), hairy cell leukemia (HCL), and all types of B-cell non-Hodgkin'' lymphoma (B-NHL).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cell differentiation antigen 37; GP52 40; Tetraspanin-26; Tspan-26; TSPAN26
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD37
• Gene ID: 951
• NCBI Gene ID: 166556
• UniProt: P11049
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Spleen cells of a patient with hairy cell leukemia
• Target Antigen: CD37
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: IPO-24
• Conjugation: Purified, BSA-free
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | Flow, surface (published) | IF (published) | IHC, FFPE (published)
• Validated Applications: FC, FC, IF, IHC, FFPE
• Field of Research: Cancer
• Positive Control: Daudi, Raji, Namalva, Ramos cells and human lymphocytes. Human tonsil and lymph node.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 33-55 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Blood (2010) 116(7): 1025-1034. (IHC, FFPE; IF) PLoS One 12(10): e0185940. (Flow, surface; IF)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years