Anti-CD35 / CR1(E11), CF640R conjugate

• Description:

  Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21 /CD35 /CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CR1

• Gene ID:

  1378

• NCBI Gene ID:

  334019

• UniProt:

  P17927

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Baboon, Cynomolgus, Human, Rhesus macaque

• Immunogen:

  Intact human monocytes

• Target Antigen:

  CD35 | CR1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  E11

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow, surface (published) | ELISA (published) | IHC, FFPE (verified) | WB (published)

• Validated Applications:

  FC, ELISA, IHC, FFPE, WB

• Field of Research:

  Immunology

• Positive Control:

  Follicular dendritic cells (FDC) in tonsil

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  210-220 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (1999) 162:7549-7554. (WB) Clin Exp Immunol (2005) 143: 274-280. (ELISA) Int Immunol (2013) 25(1): 25-33. (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years