Anti-CD147(8D6), CF640R conjugate

• Description:

  This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  BSG

• Gene ID:

  682

• NCBI Gene ID:

  501293

• UniProt:

  P35613

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human T cell line Molt 13

• Target Antigen:

  CD147

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  8D6

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Developmental biology

• Positive Control:

  HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  35 kDa (non-reduced); 40 kDa (reduced)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1:100-1:200|Inhibits homotypic aggregation, adhesion to matrix, and migation thro ugh matrigel, order Ab without BSA/azide|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years