Anti-Interferon gamma (IFNG)(G-23), CF405S conjugate

• Description: This antibody recognizes a protein of 20-25 kDa, identified as human interferon gamma (IFN-γ). Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers. T lymphocytes and NK cells mainly produce IFN-γ. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-γ (type I interferon).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: AMCF1; Beta thromboglobulin like protein; CXC chemokine ligand 8 (CXCL8); Emoctakin; Granulocyte chemotactic protein 1 (GCP1); IFG; IFI; IFN Immune; IFN-gamma (IFNG); Interleukin 8; Interferon gamma; LECT; LUCT; Lymphocyte derived neutrophil activating factor; LYNAP; Macrophage Activating Factor (MAF); Monocyte derived neutrophil activating protein (MONAP); Monocyte derived neutrophil chemotactic factor (MDNCF); Neutrophil activating factor (NAF); Neutrophil activating peptide 1 (NAP1); Neutrophil activating protein 1 (NAP1); SCYB8; T cell chemotactic factor; T Cell Interferon; Type II Interferon
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IFNG
• Gene ID: 3458
• NCBI Gene ID: 856
• UniProt: P01579
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified recombinant human IFN-gamma
• Target Antigen: Interferon Gamma
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: G-23
• Conjugation: CF405S
• Source: Animal
• Applications: IHC, FFPE (published) | IHC, frozen (published)
• Validated Applications: IHC, FFPE, IHC,
• Positive Control: PMA stimulated human peripheral blood mononuclear cells.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 20-25 kDa
• Additionnal Information: Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Dermatol (2011) 21(1): 53-7. (IHC, frozen) Infection Immunity (2005) 73(7): 3923-3928. (IHC, FFPE)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years