Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Detergent)

Human / Cynomolgus Claudin-18.2 Protein, His,Twin-Strep Tag (Detergent)

• Background: Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.
• Description: Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (CL2-H5587) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2). In the region AA Met 1 - Ala 200, the AA sequence of Human and Cynomolgus Claudin-18.2 are homologus.
• Specifications: This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus. The protein has a calculated MW of 26.3 kDa.
• Expression Region: Met 1 - Ala 200
• Host: Insect Cells
• Target: Claudin-18.2
• Conjugation: Unconjugated
• Tag: N-10×His & C-Twin-Strep
• Source: Human / Cynomolgus
• Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 6 months under sterile conditions.
• Endotoxin: 1.0 EU per μg
• Purity: 80%
• Bioactivity: Immobilized Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5587) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 (Cat. No. CL2-M34) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Liquid
• Buffer: 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5
• Additives: Glycerol
• Molecular Weight: 26.3 kDa
• Shipping Conditions: Dry ice
• Storage Conditions: -70°C