Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody

• Description:

  The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.

• Abbreviation:

  R.FokI

• CAS Number:

  9007-83-4

• Swiss Prot:

  P14870 Planomicrobium okeanokoites

• Host:

  Rabbit

• Species Reactivity:

  Planomicrobium okeanokoites

• Immunogen:

  Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Rabbit polyclonal Antibody

• Applications:

  WB

• Positive Control:

  Purified restriction enzyme FokI

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:1,000-1:2,000

• Purity:

  Immunogen affinity purified.

• Form:

  Liquid

• Buffer:

  1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 66 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  No