Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18]

• Description:

  Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.

• Product Name Alternative:

  1810036L03Rik antibody DF 5L antibody DF5L antibody DFNA 5L antibody DFNA5L antibody FKSG 10 antibody FKSG10 antibody FLJ12150 antibody Gasdermin antibody Gasdermin D antibody Gasdermin domain containing 1 antibody Gasdermin domain containing protein 1 antibody Gasdermin domain-containing protein 1 antibody Gasdermin-D antibody GasderminD antibody GSDMD antibody GSDMD_HUMAN antibody GSDMDC 1 antibody GSDMDC1 antibody

• Abbreviation:

  GSDMD

• CAS Number:

  9000-83-3

• Swiss Prot:

  P57764 Human, Q9D8T2 Mouse,

• Cellular Locus:

  Cell membrane, Secreted.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein within Gasdermin D full length protein.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P, IF-Cell

• Positive Control:

  SiHa cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, L6 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours, mouse intestine tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:2,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 53/30 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes