ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]

• Description:

  The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

• Product Name Alternative:

  53BP2 antibody Apoptosis stimulating of p53 protein 2 antibody Apoptosis stimulating protein of p53 2 antibody Apoptosis-stimulating of p53 protein 2 antibody ASPP2 antibody ASPP2_HUMAN antibody BBP antibody Bcl2 binding protein antibody Bcl2-binding protein antibody NY REN 51 antigen antibody p53 binding protein 2 antibody p53-binding protein 2 antibody p53BP2 antibody PPP1R13A antibody Renal carcinoma antigen NY-REN-51 antibody Tp53bp2 antibody Tumor protein p53 binding protein 2 antibody Tumor suppressor p53 binding protein 2 antibody Tumor suppressor p53-binding protein 2 antibody

• Abbreviation:

  TP53BP2, Bbp, 53BP2, p53BP2

• CAS Number:

  9000-83-3

• Swiss Prot:

  Q13625 Human, Q8CG79 Mouse

• Cellular Locus:

  Nucleus, perinuclear region.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Synthetic peptide within N-terminal human ASPP2.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IF-Cell, IF-Tissue, IHC-P

• Positive Control:

  Hela cell lysate, MCF-7 cell lysate, Hela, 293T, human kidney tissue, human prostate tissue, mouse testis tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:100-1:500

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  150 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes