NEDD4L Recombinant Rabbit Monoclonal Antibody [SN73-03]

• Description:

  NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGFβ RII, NEDD4-L is thought to inhibit the TGFβ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events.

• Product Name Alternative:

  E3 ubiquitin protein ligase NEDD4 like protein antibody E3 ubiquitin-protein ligase NEDD4-like antibody KIAA0439 antibody NED4L_HUMAN antibody NEDD4 2 antibody NEDD4-2 antibody NEDD4 2c antibody Nedd4-2 antibody NEDD4-2a antibody NEDD4.2 antibody NEDD4b antibody NEDD4L antibody NEDD4La antibody NEDD4Lb antibody NEDD4Lc antibody NEDD4Ld antibody NEDD4Le antibody NEDD4Lf antibody NEDD4Lg antibody NEDD4Lh antibody NEDL3 antibody Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody Neural precursor cell expressed, developmentally down regulated 4 like antibody RSP5 antibody Ubiquitin protein ligase Rsp5 antibody

• Abbreviation:

  NEDD4L

• CAS Number:

  9000-83-3

• Swiss Prot:

  Q96PU5 Human, Q8CFI0 Mouse

• Cellular Locus:

  Cytoplasm, Endosome, Golgi apparatus.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within Human NEDD4L aa 926-975 / 975.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IP

• Positive Control:

  HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:1,000-1:5,000 ;IP: Use at an assay dependent concentration.

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 112 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes