Mouse CD117 / c-kit Protein, Fc Tag (MALS verified)

• Background:

  Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117, which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

• Description:

  Mouse CD117, Fc Tag (CD7-M5259) is expressed from human 293 cells (HEK293) . It contains AA Ser 25 - Thr 519 (Accession # P05532-2) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 81.5 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P05532-2

• Expression Region:

  Ser 25 - Thr 519

• Host:

  HEK293

• Target:

  CD117

• Conjugation:

  Unconjugated

• Tag:

  C-Fc

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human SCF protein, His Tag at 2 μg/mL (100 μL/well) can bind Mouse CD117, Fc Tag (Cat. No. CD7-M5259) with a linear range of 0.078-5 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  81.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Mouse