AAV2 VP3, Recombinant Protein

• Background:

  Adeno-associated virus is a single-stranded DNA virus and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid (CAPside) and a 4.7 KB length single stranded DNA genome. The protein capsid consists of three subunits, VP1, VP2, and VP3.

• Description:

  AAV2 VP3, Recombinant Protein (VP3-A5145) is expressed from E. coli cells. It contains AA Met 1 - Leu 533 (Accession # P03135-3) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 62.1 kDa. The protein migrates as 55 kDa and 58-60 kDa under reducing (R) condition (SDS-PAGE) .

• CAS Number:

  9000-83-3

• Accession Number:

  P03135-3

• Expression Region:

  Met 1 - Leu 533

• Host:

  E. coli

• Target:

  VP3

• Conjugation:

  Unconjugated

• Tag:

  N-10xHis

• Source:

  Adeno-associated virus 2

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized AAV2 VP3, Recombinant Protein (Cat. No. VP3-A5145) at 1 μg/mL (100 μL/well) can bind Anti-AAV,VP1/VP2/VP3,B1 Mouse Monoclonal with a linear range of 0.1-6 ng/mL (QC tested).

• Format:

  Liquid

• Buffer:

  20 mM Tris, 0.5 M NaCl, 0.5 M Arginine, pH8.0

• Additives:

  Trehalose

• Molecular Weight:

  62.1 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  500ug*1

• Species:

  Adeno-associated virus 2