Anti-CD3e (T-Cell Marker)(rC3e/1308), CF647 conjugate

• Description:

  Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD3 epsilon; CD3 TCR complex; CD3e antigen epsilon polypeptide (TiT3 complex); T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD3E

• Gene ID:

  916

• NCBI Gene ID:

  3003

• UniProt:

  P07766

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human CD3E protein

• Target Antigen:

  CD3e

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  rC3e/1308

• Conjugation:

  CF647

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Positive Control:

  Jurkat cells. Tonsil and lymph node

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  20 kDa

• Additionnal Information:

  Not recommended for flow cytometry|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Western blotting 1-2 ug/mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years