Anti-WIPI2(2A2), CF405S conjugate

• Description:

  WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. , 2004 ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WIPI2; WIPI49 like protein 2

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  WIPI2

• Gene ID:

  26100

• NCBI Gene ID:

  122363

• UniProt:

  Q9Y4P8

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant fragment (around aa 418-436) of human WIPI2 protein (exact sequence is proprietary)

• Target Antigen:

  WIPI2

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  2A2

• Conjugation:

  CF405S

• Source:

  Animal

• Applications:

  IF (published) | IP (published) | WB (published)

• Validated Applications:

  IF, IP, WB

• Positive Control:

  HEK293 cells. Human or mouse placenta, skeletal muscle or testis.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  49 kDa

• Additionnal Information:

  ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Autophagy (2010) 6(4):506-522. (IF) Brain (2019) 142(5): 1242-1254. (IF, IP, WB)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years