Anti-HLA-DP (MHC II)(HLA-DPB1/2862R), CF640R conjugate

• Description:

  Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  HLA class II histocompatibility antigen DP alpha 1 chain; HLA DP1A; HLA SB alpha chain; HLADP; HLADPA1; HLASB; HLASB histocompatibility type; Human MHC class II HLA SB alpha; Major histocompatibility complex class II DP alpha 1; MHC class II DP3 alpha; MHC class II DPA1; PLT1; Primed lymphocyte test 1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HLA-DPB1

• Gene ID:

  3115

• NCBI Gene ID:

  347270

• UniProt:

  P04440

• Cellular Locus:

  Plasma membrane

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

• Target Antigen:

  HLA-DP

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  HLA-DPB1/2862R

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified)

• Validated Applications:

  FC, IF

• Positive Control:

  Raji cells. Tonsil or lymph node

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  33-35 kDa

• Additionnal Information:

  ELISA: For coating use Ab at 2-4 ug/mL, order Ab without BSA; Flow cytometry: 1-2 ug/million cells; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years