Anti-SIGLEC1 / CD169 / Sialoadhesin(HSn 7D2), CF594 conjugate

• Description:

  Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1, 709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD169; CD169 antigen; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Siglec-1; SN

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SIGLEC1

• Gene ID:

  6614

• NCBI Gene ID:

  31869

• UniProt:

  Q9ZZ2

• Cellular Locus:

  Plasma membrane|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.

• Target Antigen:

  CD169 | Sialoadhesin | SIGLEC1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  HSn 7D2

• Conjugation:

  CF594

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IHC, FFPE (published) | WB (published)

• Validated Applications:

  FC, IHC, FFPE, WB

• Positive Control:

  Human spleen, human peripheral blood monocytes or MCF7 cell.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  185 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Sci (2013) 104(9): 1237-1244. (WB; cell ELISA; IHC, FFPE) Rheumatology (2014) 53(2): 250-259. (Flow, surface; functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years