Anti-ERCC1 / RAD10 (Tumor Progression Marker)(ERCC1/2683), CF568 conjugate

• Description:

  Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  COFS4; DNA excision repair protein ERCC1; ERCC1; Excision repair cross complementing 1; RAD10; UV20

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ERCC1

• Gene ID:

  2067

• NCBI Gene ID:

  435981

• UniProt:

  P07992

• Cellular Locus:

  Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

• Target Antigen:

  ERCC1 | RAD10

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  ERCC1/2683

• Conjugation:

  CF568

• Source:

  Animal

• Positive Control:

  HeLa, HepG2 cells. Tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  ~110 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years