Anti-CD3e (T-Cell Marker)(UCHT1), CF594 conjugate

• Description:

  Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD3E

• Gene ID:

  916

• NCBI Gene ID:

  3003

• UniProt:

  P07766

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Chimpanzee, Human

• Immunogen:

  Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

• Target Antigen:

  CD3e

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  UCHT1

• Conjugation:

  CF594

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Functional studies (published) | ELISA (published) | Flow, surface (verified) | IF (verified) | IP (published) | WB (published)

• Validated Applications:

  ELISA, FC, IF, IP, WB

• Positive Control:

  Human peripheral blood. Jurkat cells. Tonsil or lymph node.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  20 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. _x000D_ 1. Blood (1988) 71(3): 603-612. (immunofluorescence, western)_x000D_ 2. J Immunol Meth (1988) 106(1): 127-133. (ELISA)_x000D_ 3. J Exp Med (1991) 173: 7-17. (immunoprecipitation)_x000D_ 4. J Immunol (2002) 168: 3235-3241. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years