Anti-CD45 / LCA (B-Cell Marker)(rPTPRC/1460), CF640R conjugate

• Description:

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  B220; CD45R; GP180; Leukocyte common antigen (LCA); Loc; Ly-5; Lyt-4; Protein tyrosine phosphatase receptor type C (PTPRC); Receptor-type tyrosine-protein phosphatase C; T200 glycoprotein

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  PTPRC

• Gene ID:

  5788

• NCBI Gene ID:

  654514

• UniProt:

  P08575

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human recombinant full-length CD45 protein

• Target Antigen:

  CD45 | LCA

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  rPTPRC/1460

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Field of Research:

  Immunology, Signal transduction

• Positive Control:

  Ramos, U-698 or GA-10 cells. Tonsil and Spleen.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  180-220 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years