ABTS 1-Component HRP Microwell Substrate (SUBA)

• Description:

  ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors.

• Target Antigen:

  Apoptosis

• Assay Principle:

  1. Run ELISA according to the specific protocol through the conjugate incubation step., 2. Wash the wells three or four times with 1X ELISA Wash Buffer (catalog #652) to remove any residual, AP-conjugate., 3. Bring SUBP to room temperature; protect from light., 4. Pipette 100 µL SUBP into each well of the plate., 5. Incubate SUBP 10-60 minutes. Monitor the color intensity., 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 µL/well STOPP (catalog #6284) and read the samples between 405-420 nm within 1 hour.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C

• Shelf Life:

  36 months from date of manufacture