Rat β-CTx (Beta Crosslaps) ELISA Kit

Rat β-CTx (Beta Crosslaps) ELISA Kit

• Abbreviation: β-CTx
• Synonyms: B-Cr;BCL;BCTX-I;BCTXI;Type I Collagen C-Telopeptide-Related Fraction;b-CTx
• CAS Number: 7732-18-5
• UniProt: PN/A2454
• Species Reactivity: Rat
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: Signal transduction;Metabolism
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat β-CTx. During the reaction, Rat β-CTx in the sample or standard competes with a fixed amount of Rat β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 2 h 30 min
• Detection Range: 125-8000 pg/mL
• Sensitivity: 75 pg/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C/-20°C, 12 months