Mouse Stromal Cell-Derived Factor-1 beta Recombinant

• Background: SDF-1alpha and SDF-1beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophilsin vitroand is a highly potent chemoattractant for mononuclear cellsin vivo. In addition, SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34 (+) cells in response to SDF is increased by IL-3in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.
• Description: Recombinant murine stromal cell-derived factor-1beta (SDF1β), also known as CXCL12 or Pre-B cell growth-stimulating factor (PBSF), is a disulfide-linked monomeric protein consisting of 73 a.a. and migrates as an approximately 8 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding mouse Stromal Cell-Derived Factor-1 beta (SDF1β) mature chain was expressed inE. coli.
• UniProt: P40224
• Endotoxin: < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 97% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 8 kDa
• Formulation: Lyophilized from a 0.2 µm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween-80,5 mM glutamic acid, pH 4.5.