Human S100 Calcium Binding Protein A4 Recombinant

• Background:

  S100A4 is a calcium-binding protein related to the S100 family of proteins. It is known also as a growth-related protein, CAPL. It is expressed at high levels in different mouse metastatic cells and in normal tissues (thymus, spleen) and activated macrophages, T-cells, neutrophils, monocytes, and macrophages.

• Description:

  Recombinant S100-A4 is a disulfide-linked homodimer protein consisting of 332 amino acid residues (containing a C-terminal IgG1 Fc tag), and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human S100-A4 mature chain was expressed inE. coli.

• UniProt:

  P26447

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 95% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  12 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered PBS solution.