Mouse Eotaxin Recombinant

• Background:

  Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophilsin vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils .

• Description:

  Recombinant mouse Eotaxin is a disulfide-linked homodimeric protein consisting 75 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Mouse Eotaxin mature chain was expressed inE. coli.

• UniProt:

  P48298

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 95% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  8 kDa

• Formulation:

  Lyophilized from 0.2 µm filtered PBS solution, pH 7.4.