Spike Trimer (S1+S2) (B.1.1.7, Alpha Variant), His-Tag (SARS-CoV-2) Recombinant

• Description:

  Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.1.7, also known as variant Alpha originally discovered in the United Kingdom. It contains mutations N501Y, A570D, D614G, P681H, T716I, S982A, D1118; deletions: 21765:6 (69-70HV), 21991:3 (144Y) . The construct also contains mutations 682RRAR685>A, K986P and V987P, and a T4 trimerization domain followed by a His-tag (6xHis) in C-terminal. The recombinant protein was affinity purified.

• UniProt:

  P0DTC2

• Tag:

  C-terminal His-tag

• Purity:

  ≥ 90%

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  137 kDa

• Formulation:

  8 mM phosphate pH 7.4,110 mM NaCl, 2.2 mM KCl, 20% glycerol.

• Glycosylation:

  This protein runs at a higher MW by SDS-PAGE due to glycosylation.